Journal: Respiratory Research

Article Title: Expression profiling identifies genes involved in emphysema severity

doi: 10.1186/1465-9921-10-81

Figure Lengend Snippet: Comparison of mRNA expression in seven candidate genes between TPCH test (n = 30, microarray) and training set (n = 62 qRT-PCR data), with two public microarray datasets of lung tissue samples (Spira et al , , n = 34; and Golpon et al , n = 10) . Fold change represents mean expression ratio of moderate versus mild emphysema (TPCH training set), severe/mild emphysema versus normal (Spira et al ), or severe emphysema versus normal samples (Golpon et al ). The absence of a bar indicates the gene was not represented on the microarray platform.

Article Snippet: Lung and universal reference RNA (Stratagene, La Jolla, CA, USA) was reverse transcribed, labeled with Cy5 and Cy3 (Amersham/GE Healthcare, Buckinghamshire, England) respectively and co-hybridized onto a 22K Operon V2.1 Human Genome Oligo Microarray chip http://www.operon.com containing 21,329 70 mer probes representing ~14,200 named transcripts printed by the British Columbia Gene Array Facility http://www.microarray.prostatecentre.com .

Techniques: Expressing, Microarray, Quantitative RT-PCR

Journal: Experimental and Therapeutic Medicine

Article Title: Altered expression levels of IDH2 are involved in the development of colon cancer

doi: 10.3892/etm.2012.676

Figure Lengend Snippet: Gene expression in colon carcinoma samples compared to adjacent peritumor samples as determined by microarray. The red signals represent higher expression of genes and the green signals represent lower expression of genes.

Article Snippet: A cDNA microarray chip (22K Human Genome Array Chip; CapitalBio, Co., Ltd., Beijing, China) was used for the tumor or peritumor tissue samples in this study.

Techniques: Gene Expression, Microarray, Expressing

Journal: PLoS ONE

Article Title: Growth Differentiation Factor 15 Is Induced by Hepatitis C Virus Infection and Regulates Hepatocellular Carcinoma-Related Genes

doi: 10.1371/journal.pone.0019967

Figure Lengend Snippet: Huh7.5.1 cells were infected with JFH-1 HCVcc (HCV, MOI = 1) or the same amount of UV-inactivated virus (HCVUV) for 2 to 4 days (2–4 D). Total RNA was isolated for microarray and quantitative real-time RT-PCR analysis. ( A ) GDF15 mRNA expression abundances were extracted from microarray raw data and represented as fold increases. ( B ) GDF15 mRNA levels were determined by real-time RT-PCR in the same manner as experiments designed in microarray analysis. ( C ) Concentrations of GDF15 protein in the medium supernatants sampled on cultured Huh7.5.1 cells 3 days post HCV or HCVUV infection. ( D ) Huh7.5.1 cells seeded in a 24-well plate were transfected with 1.5 µg of plasmid DNA, expressing the indicated viral proteins or an empty vector alone as a control. Three days after transfection, the levels of secreted GDF15 in culture supernatants were quantified by the ELISA method. ( E ) By immunobloting, the successful expression of above Flag-tagged individual viral proteins was confirmed. The position of each protein was indicated with a star sign.

Article Snippet: The microarray hybridization and analysis were performed by CapitalBio Corporation (Beijing, China) using the 22K Human Genome Array.

Techniques: Infection, Virus, Isolation, Microarray, Quantitative RT-PCR, Expressing, Cell Culture, Transfection, Plasmid Preparation, Control, Enzyme-linked Immunosorbent Assay, Western Blot